Fig. 7

HUMSC-Exos activated PTEN/mTOR/TGF-β1 signaling to promote tendon repair by delivering miR-29a-3p. A Base sequence of hsa-miR-29a-3p and illustration of luciferase reporter gene containing the wild-type and mutant 3′UTR region of PTEN with binding sequence of hsa-miR-29a-3p. B Relative luciferase activity after transfection. C Representative western blot images of PTEN, TGF-β1, p-AKT, AKT, p-mTOR and mTOR. D–G Quantification of the relative gray level of the PTEN, TGF-β1 and relative ratio of p-AKT/AKT, p-mTOR/mTOR. H Representative western blot images of COL1A1, COL3A1, DCN, and SCXA. I–L Quantitative analysis of COL1A1, COL3A1, DCN, and SCXA (n = 3 per group). *P < 0.05, **P < 0.01, ***P < 0.001. ns not significant