Fig. 4

Uptake and targeting abilities of ICG/PTX@RGE-EV were identified in vitro. A Expression level of NRP-1 in U251 and Bel-7404 cells detected by Western blot analysis. B Fluorescence intensity of ICG in different groups. C ICG-positive cell percentage detected by flow cytometry. Measurement data were expressed as mean ± standard deviation. Data comparison was analyzed by one-way ANOVA among multiple groups, followed by Tukey’s post hoc test. *p < 0.05. The experiment was repeated 3 times independently