Fig. 3
From: Autophagy deficiency exacerbates acute lung injury induced by copper oxide nanoparticles
CuONPs instillation caused inflammation in lung tissues of mice. Mice were intratracheally instilled with vehicle, 1.5, 2.5 or 5 mg/kg CuONPs, respectively for 3 days. The mRNA expression level of A Il-6, B Il-1β and C Tnf-α in mice lung tissues in the indicated groups were determined by QPCR analysis. D The expression levels of CD68 in lung tissues in the indicated groups were determined by IHC analysis. Scale bar, 100 μm in the upper panels and 50 μm in the bottom panels. E The average of CD68 intensity shown in D were quantified. F The mRNA expression levels of Ccl2 and Sele in lung tissues in the indicated groups were determined by QPCR assay. *indicates p < 0.05 compared with vehicle group