Fig. 8
From: Autophagy deficiency exacerbates acute lung injury induced by copper oxide nanoparticles
Loss of lc3b aggravated lung injury induced by CuONPs. Wild type and lc3b-/- mice were intratracheally instilled of vehicle or 5 mg/kg CuONPs, respectively, then the lung tissues and BALFs of indicated groups were collected 3 days after treatment. A, B The protein expression levels of LC3B-I/II in the mice lung tissues were determined by western blot analysis. β-actin was served as internal control and the indicated protein expression levels were quantified. C Representative H&E staining images of lung tissues. Scale bar, 200 μm in the upper panels and 50 μm in the bottom panels. D The lung injury scores of H&E staining were calculated. E The cell counts in BALFs collected from indicated groups were determined by automated cell counter. F The protein concentrations of BALF collected from the indicated groups were determined by BCA analysis. G The LDH activities of BALFs collected from indicated groups were determined. *indicates p < 0.05 compared with indicated group