Fig. 3

HP-SEC analysis of HumFt-PAMAM nanoparticle. G4 PAMAM was labelled with FITC and encapsulated into HumFt protein shell. The hybrid nanoparticle formation was monitored following the fluorescence signal (green line) of G4 PAMAM/FITC (ex 490 nm, em 525 m) at the retention time (4.5 min) of ferritin 24-mer (UV chromatogram, blue line). Empty ferritin does not show intrinsic fluorescence upon excitation at 490 nm (red line)