Fig. 6

miRNA-145-5p delivery by the HumFt-PAMAM nanoparticle promotes differentiation of the NB4 cell line. A NB4 cells were incubated with the HumFt-PAMAM-pre-miRNA-145-5p nanoparticle for 48 h. qRT-PCR revealed high levels of miRNA-145-5p (p < 0.01) and increased expression of RARα mRNA. (mean ± SEM, n = 2, p < 0.05, one-way ANOVA statistical analysis relatively to control). B Morphological analysis of NB4 cells treated as in (A) evidenced that miRNA-145-5p promotes differentiation, as indicated by cytosol enlargement, Golgi expansion, and decreased basophilia compared to control cells or to cells treated with the HumFt-PAMAM nanoparticle without pre-miRNA-145-5p (left panel). The right panel reports the ratio between the area of nucleus and cytosol (n ≥ 60 ± SEM cells from at least two different fields; statistical analysis one way ANOVA)