Fig. 7

Exosomal miR-1260a derived from BMSC-Fe₃O₄-SMF-Exos regulates HDAC7 and COL4A2. (*) p < 0.05, (**) p < 0.01, (***) p < 0.001, ns = no significance; wt, wild-type; mut, mutant; NC, negative control. a Volcano plot of miRNA sequencing analysis of mRNAs with a ≥ 1.5-fold difference in expression between BMSC-Exos and BMSC-Fe₃O₄-SMF-Exos. Green and red indicate downregulation and upregulation, respectively. b Comparison of the top five elevated miRNAs (miR-143-3p, miR-23a-3p, miR-1260a, let-7b-5p and miR-3960) between BMSC-Exos and BMSC-Fe₃O₄-SMF-Exos using qRT-PCR. c Confirmation of the transfection efficiency of miR-1260a in HUVECs and BMSCs. d The miR-1260a binding sequence in the 3′-UTR of HDAC7 and COL4A2. e Luciferase readout from wt or mut HDAC7 3′-UTR co-transfected in BMSCs (left panel) or COL4A2 3′-UTR reporter co-transfected in HUVECs (right panel) with control mimics or miR-1260a mimics. The miR-1260a mimics transfection reduces luciferase activity when compared to control mimics transfection, which confirms that HDAC7 and COL4A2 are the target genes of miR-1260a. f The protein expression of HDAC7 and COL4A2 after transfection of cells with miR-1260a mimics, miR-1260a inhibitor and their NCs. g Transfection with the miR-1260a mimics resultes in a significant decrease in the levels of HDAC7 in BMSCs (left panel) and in the levels of COL4A2 in HUVECs (right panel)