Fig. 8

Exosomal miR-1260a promotes osteogenesis and angiogenesis by targeting HDAC7 and COL4A2. (**) p < 0.01, (***) p < 0.001, ns = no significance; NC, negative control. a The red staining indicative of mineralization is most obvious when BMSCs are cotransfected with miR-1260a mimics and pcDNA-NC, while matrix mineralization is significantly reduced when the BMSCs are cotransfected with miR-NC and pcDNA-HDAC7. b Quantitative analysis indicates overexpression of HDAC7 prevents the enhancement of osteoblast differentiation in BMSCs by miR-1260a mimics. c The scratch wound assay showing the areas of wound healing among different groups. d Quantitative analysis indicates overexpression of COL4A2 prevents the upregulation of the wound recovery rate of HUVECs by miR-1260a mimics. e The transwell assay showing the cell migration among different groups. f Quantitative analysis indicates overexpression of COL4A2 suppresses the upregulation of the migration rate of HUVECs by miR-1260a mimics. g Western blotting assays showing that overexpression of HDAC7 prevents the upregulation of OPN, Runx2, OCN, ALP and COL-1 protein expression by miR-1260a mimics. h Western blotting assays showing that overexpression of COL4A2 prevents the upregulation of VEGF, ANG-1 and HIF-1α protein expression by miR-1260a mimics