Table 1 Determination of anti-MPO with different concentrations in normal serum samples by the proposed ECL inmmunosensor
Sample | Standard solution (fg mL− 1) | ECL intensity (a.u.) | Calculated (fg mL− 1) | Recovery (%) | RSD (%) |
|---|---|---|---|---|---|
1 | 50.0 | 2486.7 | 47.77 | 95.54 | 0.84 |
2 | 500.0 | 5631.3 | 505.78 | 101.16 | 3.20 |
3 | 5.00 × 103 | 8727.0 | 5.16 × 103 | 103.23 | 2.74 |
4 | 7.50 × 104 | 12260.3 | 7.32 × 104 | 97.54 | 2.31 |
5 | 2.00 × 105 | 13,683 | 2.13 × 105 | 106.37 | 0.92 |