Fig. 1
Physicochemical characterization of BDNF-PAMAM as well as PEG-ylated BDNF-PAMAM nanoparticles with positively and negatively charged dendrimers core. Serum stability of nanoparticles studied using DLS. Particle size distribution (A) and hydrodynamic diameter (B) of BDNF-PAMAM5.5, BDNF-PAMAM5.5-PEG, BDNF-PAMAM6, BDNF-PAMAM6-PEG nanoparticles, measured against PAMAM dendrimers core nanoparticles (without BDNF). All samples were incubated in PBS with 10% FBS over 30 days. All experiments were performed in triplicates and error bars represent mean ± standard deviation (SD) (A and C). The dependence of the zeta potential of positively charged PAMAM 6 or negatively charged PAMAM5.5 dendrimers core on the initial BDNF concentration of 0.1 mgL−1 in the suspension in PBS, pH 7.4, 0.15 M ionic strength (C). Representative AFM “high” diagrams (B) of BDNF-PAMAM5.5, BDNF-PAMAM5.5-PEG, BDNF-PAMAM6, BDNF-PAMAM6-PEG nanoparticles adsorbed at mica surface at 0.15 M pH 7.4 in PBS at a scan area of 0.5 × 0.5 µm and structure of PAMAM-based nanoparticles after cross-section