Fig. 6

Biotinylated EV captured by tetraspanins and anti-biotin. a Biotinylated SK-OV-3 EVs (“B-EV”) were compared to PBS, free biotinylation agent (“Biotin”), and non-biotinylated SK-OV-3 EVs (“EV”). N = 3 experimental replicates, representing 3 capture spots each These results indicate that biotinylation did not majorly alter tetraspanin capture (i.e., “B-EV” compared to “EV”) in most cases. For biotinylated (b) serum and (c) PMSC derived EVs, some "multiplex bias” could be observed, e.g., CD9 captured by single-tetraspanin compared to non-specific capture. N = 3 capture spots. Error bars represent ± one s.d. * p < 0.05, ** p < 0.01, *** p < 0.001 by ANOVA