Fig. 4

HH protects BADSCs against anoikis in vitro and in vivo. a HH promoted the adhesion of BADSCs in vitro against ROS (H₂O₂ was used to provide ROS); b, c HH protected BADSCs in vitro against ROS after adhesion; d schematic illustrating the protection of HH on BADSCs against ROS; e quantitative analysis of adhered cells on different substrate under treatment of 100 µM H₂O₂ for 6 h. H₂O₂ was added immediately after cell seeding; f quantitative analysis of adhered cells on different substrate under treatment of 100 µM H₂O₂ for 6 h. H₂O₂ was added after cell adhesion (12 h after cell seeding); g analysis of cell adhesion and extension under treatment of 500 µM H₂O₂ for 6 h. H₂O₂ was added after cell adhesion (12 h after cell seeding); h In situ retention of BADSCs after intramyocardial injection in PBS and in HH; h, schematic illustrating the mechanism of cell retention in situ after delivery in HH; i Quantitative analysis of cell retention in recipient