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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: The impact of anionic polymers on gene delivery: how composition and assembly help evading the toxicity-efficiency dilemma

Fig. 5

Toxicity of polyplexes in HEK293T cells. The cells were treated with (layered) polyplexes at N*/P 30 for 24 h. Incubation with pDNA only was used as control. Subsequently, the viability of the cells was investigated using A the PrestoBlue assay for metabolic activity, B the LDH release assay, or C flow cytometry with gating for viable cells in the FSC/SSC plot. Cells treated with pDNA only served as control. Values represent mean ± SD (n = 3). */**/***Significant differences to control (p < 0.05/0.01/0.001)

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Journal of Nanobiotechnology

ISSN: 1477-3155

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