Fig. 4

BMEVs stimulate ROS generation and downregulate NLRP3 expression. a KEGG network analysis of CAL 27 cells after BMEVs treatment at 6 h; b KEGG network analysis of CAL 27 cells after BMEVs treatment at 12 h; c Western blot analysis of JUN expression after BMEVs treatment; d fluorescence images of the intracellular ROS levels using DCFH-DA, scale bar = 200 μm; e cell viability of CAL 27 and WSU-HN 6 cells treated with BMEVs and scavenger NAC and CH; f Western blot analysis of JUN expression after BMEVs treatment with NAC or CH; g Western blot analysis of NLRP3 and pro-IL-1β expression after BMEVs treatment; h The IL-1β concentration of CAL27 cultured medium pre- and post-BMEVs treatment; i microRNAs extracted from BMEVs; j RT-PCR assay of relative expression of NLRP3 in CAL 27 and WSU-HN6 cells treated with BMEVs derived RNAs