Fig. 5

Cell viabilities of 4T1 cells after different treatments in normoxic (a) and hypoxic (b) conditions. Data shown as mean ± SD, n = 3 per treatment. Statistical significance was set at *p < 0.05, **p < 0.01, ***p < 0.001. c The calcein AM/PI co-stained images of 4T1 cells after different treatments in both normoxic and hypoxic conditions. Scale bar = 50 μm. d PA values as a function of APCZ concentration. Insert shows the corresponding in vitro PA images. e In vivo PA images of the tumor site after tail intravenous injection of APCZ dispersion for different time periods (0, 2, 5, 10, 16, 24 h). f Average PA values at tumor site corresponding to e. Data shown as mean ± SD, n = 3 per treatment