Table 1 Ligation efficiency of antibodies in different immunoliposomes
Connection method | molar ratio | Added Protein content (μg/mL) | Measured Protein content (μg/mL) | Connection efficiency (%) |
|---|---|---|---|---|
Amide bond | 1:25 | 3.2 | 0.083 | 2.60 ± 2.10 |
1:50 | 1.6 | 0.343 | 21.43 ± 3.44 | |
1: 100 | 0.8 | 0.692 | 86.53 ± 6.78 | |
1: 200 | 0.4 | 0.316 | 78.90 ± 6.15 | |
1: 400 | 0.2 | 0.150 | 74.84 ± 7.04 | |
Disulfide bond | 1:25 | 3.2 | 0.0154 | 0.48 ± 0.22 |
1:50 | 1.6 | 0.015 | 0.28 ± 0.13 | |
1: 100 | 0.8 | 0.004 | 1.38 ± 1.01 | |
1: 200 | 0.4 | 0.011 | 1.94 ± 0.89 | |
1: 400 | 0.2 | 0.008 | 3.33 ± 2.33 |