Fig. 10

In vivo effect of free MTM and MTM-LIP. T-5 H-FC#1 established xenografts were randomly assigned to five different groups (n = 5 per group) and treated intravenous with vehicle (PBS, control), free MTM at 1 or 2 mg/kg or MTM-LIP at 1 or 2 mg/KG every 3–4 days (twice a week). A Curves representing the mean relative tumour volume of T-5 H-FC#1 xenografts during the treatments. Drug efficacy expressed as the percentage of TGI is indicated. B Distribution of tumour volumes at the end of the experiment (day 20 after the start of the treatment). C Change in the body weights of mice during the treatments. D H&E staining of formalin-fixed paraffin embedded livers extracted at the experimental end-point. Areas presenting microvesicular steatosis (S), necrotic cells (yellow arrows) and mitosis (grey arrows) are indicated. Scale bars = 30 μm. Error bars represent the SEM and asterisks indicate statistically significant differences between groups in one-way ANOVA Turkey’s tests (*:p < 0.05; **:p < 0.01; ***:p < 0.001; ****:p < 0.001)