Fig. 4

Antiproliferative effects induced by free and nanocarrier delivered MTM in sarcoma. A–B Cell viability (WST1 assay) measured after the treatment of T-5 H-FC#1 (A) and T-CDS17#4 (B) cells with increasing concentrations of free MTM or MTM loaded in polymeric nanoparticles (MTM-NPs), liposomes (MTM-LIP) and hydrogels (MTM-HG) for 72 h. IC₅₀ values for each condition are shown. C–F Colony formation unit (CFU) assays in of T-5 H-FC#1 (C–D) and T-CDS17#4 (E–F) cells treated with increasing concentrations of the indicated MTM formulations for 24 h and left to form CFUs for 10 days. Summary graphics (C and E) and representative pictures of a colony formation assay (D and F) for each cell type are shown. Error bars represent the standard deviation of at least three independent experiments