Fig. 8

Signaling pathways altered by the treatment with free and nanocarrier delivered MTM. RNAseq data were used to perform gene ontology analyses. A–B KEGG pathway analysis showing those signaling routes significantly altered (enrichment score (ES) ≤-0.5 or ≥ 0.5 and padj < 0.01; blue circles) when comparing M_F vs. control (A) and M_NP vs. control (B). Circle diameter for each pathway reflect the number of genes involved in the pathway (gene count) showing altered expression. Information for relevant upregulated (purple text) of downregulated (green text) pathways is displayed. C GSEA analysis of selected signaling pathways in M_F vs. control (left) and M_NP vs. control (right) comparisons. D Fold change expression (expressed as Log2-FC) and padj values obtained in the indicated comparisons for a panel genes known to be regulated by SP1. (* Several cadherins have been described to be targets of SP1, although CDH4 has not been described yet as a SP1-regulated gene)