Fig. 1

The identification of Exo and Exo-181b. a TEM was utilized for the observation of the morphology of Exo-NC and Exo-181b. Scale bar = 100 nm. b The identification of Exo-NC and Exo-181b by detecting their surface positive markers (CD9, Alix, and Tsg101) by western blot. c NTA analysis was used for analyzing the diameter distribution of Exo-NC and Exo-181b. d The uptake of Exo by RAW264.7 detected by fluorescence staining. Green, red and blue represent the cytoskeleton (phalloidine), Exo (PKH26) and nucleus (DAPI), respectively. e The gene expression of miR-181b in hBM-MSC and the secreted Exo overexpressing miR-181b. f The gene expression of miR-181b in RAW264.7 after treated by Exo-NC and Exo-181b. (*p < 0.05). Exo exosome without overexpressing miR-181b, Exo-181b exosome overexpressing miR-181b, TEM transmission electron microscopy, NTA nanoparticle tracking analysis