Fig. 5

PT + H + Exo-181b promoted osseointegration in vivo detected by histological evaluation. a Fluorochrome labeling analysis was applied for the evaluation of the osteointegration via the undecalcified specimens. The images in yellow, red, green and grey represented TE, AL, CA and BF respectively by laser confocal microscopy after the operation. b Van Gieson’s picrofuchsin was used for staining of the undecalcified specimens. Newly regenerated bone was stained in red. Scale bar = 500 µm. c Statistical analysis of the area of TE, AL and CA fluorescence. Scale bar = 500 µm. d The percentage area of new regenerated bone was calculated by Image J. (*p < 0.05). TE tetracycline, AL alizarin red, CA calcein, BF bright filed