Fig. 7
The inhibition of PRKCD enhanced M2 polarization of Exo-181b. a RAW264.7 cells were first treated with Ti and then treated with Exo-181bi NC + si-PRKCD NC, Exo-181bi NC + si-PRKCD, Exo-181bi + si-PRKCD NC and Exo-181bi + si-PRKCD, respectively for 24 h. The expression of PRKCD, p-AKT, AKT, and GAPDH was tested by Western blotting. b The concentrations of TNF-α, IL-6, IL-10 of the supernatants by ELISA. c The relative gene expression of CCR7, CD206, Arg-1 and iNOS was detected by qRT-PCR. d, e The representative images of the percentage of CCR7 and CD206 positive cells verified by flow cytometry analysis. f, g The quantitative analysis of the percentage of CCR7 and CD206 positive cells by flow cytometry analysis (*p < 0.05)