Fig. 2

Nano-DOX stimulated DAMPs emission from NSCLC cells. A–H Nano-DOX stimulated emission of HMGB1, CRT, HSP90 and ATP in in vitro A549 and Lewis cells. HMGB1 was assayed by ELISA. CRT and HSP90 was assayed by FACS analysis of immunofluorescent staining, and ATP assayed with a bioluminescence assay kit. FACS histogram geometric means were used to quantify mean fluorescence intensity (MFI). Values are means ± SD (n = 3, *p < 0.05). I Nano-DOX treatment resulted in increased immunohistological staining of DAMPs (CRT, HSP90 and HMGB1) in subcutaneous xenografts of Lewis cells in mice. Drug concentration was 2 μg/mL for DOX and Nano-DOX in the in vitro experiments and treatment duration was 24 h. Representative FACS dot plots for B, C, F and G were provided in Additional file 1: Figure S2