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Table 1 Results of Annexin V/FITC analysis performed by flow cytometry

From: Label-free cell based impedance measurements of ZnO nanoparticles—human lung cell interaction: a comparison with MTT, NR, Trypan blue and cloning efficiency assays

  Viable Early apoptotic Late apoptotic Necrotic
24 h
 CTR 86.6 ± 1.5 6.0 ± 0.3 4.1 ± 1.3 3.2 ± 1.0
 ZnO 1 87.0 ± 0.3 4.2 ± 0.8 4.1 ± 0.1 4.6 ± 0.3
 ZnO 5 74.5 ± 0.9 11.5 ± 1.1 8.2 ± 1.1 5.7 ± 0.9
 ZnO 20 51.3 ± 2.8 16.3 ± 1.3 23.0 ± 1.5 9.3 ± 0
 Rapamycin 80.0 ± 2.4 3,0 ± 0.8 8.7 ± 0.4 8.2 ± 2.7
 STS 72.4 ± 4.4 7.9 ± 0.5 11.9 ± 0.5 7.7 ± 3.4
48 h
 CTR 72.4 ± 1,5 19.9 ± 1.7 4.9 ± 1.5 2.7 ± 1.3
 ZnO 1 51.2 ± 5.6 20.3 ± 1.3 17.6 ± 3.0 10.8 ± 7.3
 ZnO 5 58.1 ± 5.4 33.5 ± 3.2 9.3 ± 0.3 2.2 ± 1.9
 ZnO 20 27.2 ± 2.7 17.2 ± 2.9 55.0 ± 0.4 0.6 ± 0.1
 Rapamycin 76.8 ± 2.0 4.2 ± 0.9 16.4 ± 1.6 2.5 ± 1.3
 STS 60.4 ± 4.7 9.1 ± 1.0 27.3 ± 4.5 3.1 ± 1.3
72 h
 CTR 78.6 ± 3.8 7.5 ± 1.0 7.0 ± 0.7 6.9 ± 3.5
 ZnO 1 46.5 ± 1.3 21.6 ± 1.2 21.5 ± 1.0 10.4 ± 3.5
 ZnO 5 51.2 ± 3.2 19.5 ± 2.5 23.7 ± 2.4 5.5 ± 1.6
 ZnO 20 25.9 ± 3.5 24.5 ± 3.9 40.4 ± 4.0 9.1 ± 3.4
 Rapamycin 73.5 ± 5.2 2.6 ± 0.8 13.1 ± 1.5 11.0 ± 2.5
 STS 31.3 ± 7.9 27.7 ± 2.8 35.8 ± 2.8 5.2 ± 2.2
144 h
 CTR 51.3 ± 8.6 12.1 ± 1.8 25.2 ± 4.1 11.4 ± 6.4
 ZnO 1 44.3 ± 6.3 10.2 ± 3.0 21.7 ± 2.3 23.6 ± 6.1
 ZnO 5 39.9 ± 3.0 12.6 ± 3.6 30.0 ± 1.8 17.4 ± 2.4
 ZnO 20 20.0 ± 3.8 2.0 ± 0.9 70.3 ± 6.2 7.6 ± 1.5
 Rapamycin 54.0 ± 6.1 10.9 ± 2.0 18.8 ± 3.0 16.2 ± 1.1
 STS 51.2 ± 3.1 24.0 ± 3.5 23.4 ± 0.7 1.4 ± 0.3
  1. The values are mean of the cell percentage ± standard deviation of two independent experiments