Table 1 Results of Annexin V/FITC analysis performed by flow cytometry
Viable | Early apoptotic | Late apoptotic | Necrotic | |
|---|---|---|---|---|
24 h | ||||
CTR | 86.6 ± 1.5 | 6.0 ± 0.3 | 4.1 ± 1.3 | 3.2 ± 1.0 |
ZnO 1 | 87.0 ± 0.3 | 4.2 ± 0.8 | 4.1 ± 0.1 | 4.6 ± 0.3 |
ZnO 5 | 74.5 ± 0.9 | 11.5 ± 1.1 | 8.2 ± 1.1 | 5.7 ± 0.9 |
ZnO 20 | 51.3 ± 2.8 | 16.3 ± 1.3 | 23.0 ± 1.5 | 9.3 ± 0 |
Rapamycin | 80.0 ± 2.4 | 3,0 ± 0.8 | 8.7 ± 0.4 | 8.2 ± 2.7 |
STS | 72.4 ± 4.4 | 7.9 ± 0.5 | 11.9 ± 0.5 | 7.7 ± 3.4 |
48 h | ||||
CTR | 72.4 ± 1,5 | 19.9 ± 1.7 | 4.9 ± 1.5 | 2.7 ± 1.3 |
ZnO 1 | 51.2 ± 5.6 | 20.3 ± 1.3 | 17.6 ± 3.0 | 10.8 ± 7.3 |
ZnO 5 | 58.1 ± 5.4 | 33.5 ± 3.2 | 9.3 ± 0.3 | 2.2 ± 1.9 |
ZnO 20 | 27.2 ± 2.7 | 17.2 ± 2.9 | 55.0 ± 0.4 | 0.6 ± 0.1 |
Rapamycin | 76.8 ± 2.0 | 4.2 ± 0.9 | 16.4 ± 1.6 | 2.5 ± 1.3 |
STS | 60.4 ± 4.7 | 9.1 ± 1.0 | 27.3 ± 4.5 | 3.1 ± 1.3 |
72 h | ||||
CTR | 78.6 ± 3.8 | 7.5 ± 1.0 | 7.0 ± 0.7 | 6.9 ± 3.5 |
ZnO 1 | 46.5 ± 1.3 | 21.6 ± 1.2 | 21.5 ± 1.0 | 10.4 ± 3.5 |
ZnO 5 | 51.2 ± 3.2 | 19.5 ± 2.5 | 23.7 ± 2.4 | 5.5 ± 1.6 |
ZnO 20 | 25.9 ± 3.5 | 24.5 ± 3.9 | 40.4 ± 4.0 | 9.1 ± 3.4 |
Rapamycin | 73.5 ± 5.2 | 2.6 ± 0.8 | 13.1 ± 1.5 | 11.0 ± 2.5 |
STS | 31.3 ± 7.9 | 27.7 ± 2.8 | 35.8 ± 2.8 | 5.2 ± 2.2 |
144 h | ||||
CTR | 51.3 ± 8.6 | 12.1 ± 1.8 | 25.2 ± 4.1 | 11.4 ± 6.4 |
ZnO 1 | 44.3 ± 6.3 | 10.2 ± 3.0 | 21.7 ± 2.3 | 23.6 ± 6.1 |
ZnO 5 | 39.9 ± 3.0 | 12.6 ± 3.6 | 30.0 ± 1.8 | 17.4 ± 2.4 |
ZnO 20 | 20.0 ± 3.8 | 2.0 ± 0.9 | 70.3 ± 6.2 | 7.6 ± 1.5 |
Rapamycin | 54.0 ± 6.1 | 10.9 ± 2.0 | 18.8 ± 3.0 | 16.2 ± 1.1 |
STS | 51.2 ± 3.1 | 24.0 ± 3.5 | 23.4 ± 0.7 | 1.4 ± 0.3 |