Fig. 6

R-DHLA-AuNCs-Zn downregulated production of proinflammatory cytokines after SCI. The percentage of Iba-1 (b), IL-1β (c), and double-positive Iba-1/IL-1β (i.e., both Iba-1 and IL-1β were positive) (d) were investigated by immunofluorescence analysis (a). The levels of Iba-1, IL-1β, and TNF-α were shown in the western blot (e) and the corresponding semiquantitative analysis (f). The levels of TNF-α (g) and IL-1β (h) were studied by ELISA assays. Scale bar = 100 μm. *P < 0.05;**P < 0.01. Data presented as mean ± SD. (n = 6/group)