Fig. 3 

Cellular uptake and synergistic in vitro antitumor effect of hybrid nanoassemblies. a Intracellular mechanism of HSP90 inhibition -enhanced PTT by hyperthermia sensitization; b Confocal imaging of 4T1 cells treated with DiR Sol, DG Sol, DG NPs and DG PEG_2K NPs for 0.5 and 2 h, at a DiR equivalent dose of 2 μmol/L; c Quantitative results of cellular uptake in 0.5 and 2 h from flow cytometry, respectively; d Synergistic cytotoxicity against 4T1 cells with or without laser irradiation (3 W/cm², 3 min); e Western blotting analysis of HSP90 protein expression in 4T1 cells with simulated heat treatment at 50 °C for 3 min. Scale bar = 50 μm. n.s. no significance, ***P < 0.001