Fig. 1
From: Highly sensitive and portable mRNA detection platform for early cancer detection
The principle of the CHAGE strip for mRNA detection. A In the first amplification step, H1 and H2 hairpins are designed based on the target. When the target exists, it will react with H1, enabling reaction with H2, which releases the target for further amplification. B In the second amplification step, the product from the first amplification step reacts with gold signal particles, and then trapped in the testing zone, with the control zone guaranteeing the strip’s validity. Lastly, the Au enhancer buffer (HAuCl4/NH2OH·HCl) improves the detection sensitivity via gold deposition enhancement. The strips zone illustrates positive and negative conditions