Fig. 2
From: Metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usage
Effect of methionine depletion on protein labeling and virus production. A DBCO-AF647 labeling of RD-MetRS* cells cultured in normal medium supplemented with different concentrations of ANL. Fluorescent images of RD-MetRS* cells cultured in normal or Met- medium are shown in B. Scale bar: 50 μm. C The viability of RD-MetRS* cells cultured in normal (Met+) or methionine-free medium (Met-) supplemented with ANL at different concentrations was detected by CCK-8 assay, and the titers of viruses produced by AHA-based system (Met-AHA+) and ANL-based system (Met + ANL+) are shown in D