Fig. 5
From: Metabolic labeling of enterovirus 71 with quantum dots for the study of virus receptor usage
Purification and characterization of EV71-QDs. A Image of the DGC tube after the separation of EV71-QD under bright light. B Image of fractions F1 ~ F12 under a UV lamp. C VP1 proteins in F1 ~ F12 were analyzed by Western blotting to confirm the presence of EV71. D Fluorescence spectra of DBCO-QDs and EV71-QDs. E DBCO-QDs (8 nM) and DBCO-QF647 (2.5 µM) were continuously illuminated by a laser at 488 nm or 633 nm and kinetics of the fluorescence intensity were determined by a microplate reader in four minutes. F TEM images of DBCO-QDs, EV71, and EV71-QDs. Scale bar: 50 nm. G One-step growth curve of EV71, EV71-AF647, and EV71-QDs