Fig. 2

Identification and characterization of ASA6. A Amino acid sequences of complimentary determining region of heavy chain (CDR-H) and light chain (CDR-L) of ASA6 antibody. B Image of a Coomassie brilliant blue-stained SDS-PAGE gel of purified ASA6 antibody. C Immunoblots of protein extract from human aortic plaque (AS) and Ox-LDL detected with ASA6 antibody. BSA control, AS and Ox-LDL were separated by SDS-PAGE (left), and blotted onto nitrocellulose membranes (right). D The quantitative analysis of Immunoblots. E Detection of atherosclerotic lesions in human coronary arteries by immunofluorescence with ASA6 antibody and Oil-red O staining. F The quantitative analysis of immunofluorescence staining. Data are represented as means ± s.d. from three independent replicates. ^***p < 0.001, one-way ANOVA (G) or Student’s t test (I)