Fig. 4

In vitro and in vivo biocompatibility assessment of ASA6-NPs probe. A The MTT assays of HAECs after incubated with ASA6-NPs probe for 12 and 24 h. B–D The serum levels of inflammation factors tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and C-reactive protein (CRP) in different time points after the probe injection in mouse. E–J Mouse mRNA expression levels of inflammatory cytokines TNF-α, IL-1α, IL-1β, IL-6, IL-10 and monocyte chemoattractant protein-1 (MCP-1) in liver and kidney at 7 days post-injection. K The HE staining (including heart, kidney, liver, lung, spleen, bladder, cerebral cortex and hippocampus) of mice at 7 days post-injection. L The chlorophosphonazo III staining of mouse kidney at 1 day and 7 days after injection, and their RGB value analysis. Data represent means ± s.d. from three independent replicates. NS, no significance as indicated, one-way ANOVA