Fig. 6

The therapeutic effects of ASA6 on atherosclerosis in vitro and in vivo. A Representative images of RAW 264.7 cell apoptosis detected by TUNEL staining. RAW 264.7 cells were treated with various concentrations of ASA6 and Ox-LDL. B The quantitative analysis of TUNEL positive cells. C The Dil-Ox-LDL uptake by RAW 264.7 cells. RAW 264.7 cells were treated with various concentrations of ASA6 and Ox-LDL. Blue fluorescence indicates cell nuclear. Red fluorescence indicates Dil-ox-LDL particles in RAW 264.7. D The quantitative analysis of the Dil-ox-LDL uptake. E Oil-red O staining of atherosclerotic lesions in aortic sinus in ApoE^−/− mice injected with PBS control or ASA6. F The quantitative analysis of atherosclerotic lesions. Data represent means ± s.d. from three independent replicates. ^**p < 0.01, one-way ANOVA (D) or Student’s t test (F)