Fig. 2

The toxicity of HPDA and HPDAlR. A Relative viabilities of HaCaT cells treated with vehicle (serum-free medium), HPDA (0.01 mg/mL), RL-QN15 (1 nM) and HPDA (0.01 mg/mL)lR(1 nM). Data were expressed as Mean ± SD of three independent experiments performed in quadruplicate. *P < 0.05, **P < 0.01 and ***P < 0.001 indicated statistically significant differences between the two groups (Student t-test). B Relative viabilities of RAW264.7 cells treated with vehicle (serum-free medium), HPDA (0.01 mg/mL), RL-QN15 (1 nM) and HPDA (0.01 mg/mL)lR(1 nM). Data were expressed as Mean ± SD of three independent experiments performed in quadruplicate. C The representative confocal fluorescence images of HaCaT treated with vehicle (serum-free medium), HPDA (0.01 mg/mL), RL-QN15 (1 nM) and HPDA (0.01 mg/mL)lR(1 nM). The live/cells were stained by Calcein-AM with green fluorescence and PI with red fluorescence, respectively. Scale bar: 75 µm. D H&E staining of the main organs from mice of whose dorsal skin wounds treated with vehicle (PBS), HPDA (0.1 mg/mL), RL-QN15 (1 nM), and HPDA (0.1 mg/mL)lR(1 nM). Scale bar: 100 µm