Fig. 4

Topical application of nanocomposites of MPDA + RL-QN15 accelerated healing of full-thickness skin wounds in mice. A Representative images of skin wounds on days 0, 2, 4, 6, and 8 post-injury in PBS, MPDA, RL-QN15, and nanocomposites of MPDA and RL-QN15-treated groups. MPDA (0.2 mg/mL), RL-QN15 (1 nM), and MPDA (0.2 mg/mL) + RL-QN15 (1 nM) were applied topically twice a day to skin wounds. B Quantitative curves of pro-healing activities of PBS, MPDA, RL-QN15, and MPDA + RL-QN15, respectively. C Histopathological examination of PBS, MPDA, RL-QN15 and RL-QN15 + PDA-treated full-thickness excisional wounds stained with H&E. NE, neo-epidermal; GT, granulation tissue; ES, eschar; M, muscle; Scale bar: 500 μm. Yellow line indicates neoepithelium. D, E The quantitative analysis of neo-epidermal thickness on days 4 and 8 post-operative. F, G The quantitative analysis of granulation thickness on day 4 and 8 post-operation, respectively. Dates were presented as mean ± S.D. from 9 mice (n = 9). *P < 0.05, **P < 0.01, and ****P < 0.0001 indicated statistical differences between two groups