Fig. 6

The effect of RL-QN15 against full-thickness dorsal skin wound in swine was enhanced by the load of MPDA. A Representative images of full-thickness wounds on days 0, 7, 14, 21, and 28 post-injury in PBS, MPDA, RL-QN15, and nanocomposites of MPDA + RL-QN15 groups. MPDA (0.2 mg/mL), RL-QN15 (250 nM), and MPDA (0.2 mg/mL) + RL-QN15 (250 nM) were applied topically twice a day to the wounds. B Quantitative curves of pro-healing activities of PBS, MPDA, RL-QN15, and nanocomposites of MPDA + RL-QN15. C Representative images of skin wound sections stained with H&E and Masson’s trichrome staining on days 28 post-injury in PBS, MPDA (0.2 mg/mL), RL-QN15 (250 nM), and nanocomposites of MPDA (0.2 mg/mL) + RL-QN15 (250 nM) groups. NE, neo-epidermal; GT, granulation tissue; ES, eschar; BV, blood vessels; HF, hair follicle. D The quantitative analysis of neo-epidermal thickness on day 28 post-operative. E Quantification of collagen positive area on day 28 post-operative. Data were presented as mean ± S.D. from 6 wounds (n = 6). *P < 0.05, **P < 0.01, and ****P < 0.0001 indicated statistically differences between two groups