Fig. 9

A Schematic diagram of the in vitro BBTB model established by co-cultured HUVECs and C6 cells into upper and lower chamber. B Quantitative analysis of C6 cells uptake in the lower chamber by flow cytometry after incubation with free Cou-6, Cou-6/PMs, Cou-6/Aβ-CN-PMs, rhApoE/Cou-6/Aβ-CN-PMs for 3 h, respectively. Means ± SD, n = 3; *P < 0.05, **P < 0.01, ***P < 0.001 versus the rhApoE/Cou-6/Aβ-CN-PMs group. C CLSM analysis of uptake by C6 cells in the lower chamber for 3 h incubation with free Cou-6, Cou-6/PMs, Cou-6/Aβ-CN-PMs and rhApoE/Cou-6/Aβ-CN-PMs in the upper chamber (magnification × 200)