Fig. 8

Effects of chemo-PDT combined with anti-PD-L1 (G1, PBS; G2, Anti-PD-L1; G3, TB/PTX@RTK + L; and G4, TB/PTX@RTK + L + anti-PD-L1). A Treatment schedule for combination therapy strategy of TB/PTX@RTK micelle-mediated Chemo-PDT and anti-PD-L1. B, C Primary tumor images and tumor growth curves in the different groups after the indicated treatments. (n = 5). Scale bar: 1 cm. *P < 0.05, **P < 0.01, ***P < 0.001. D, E Distant tumor images and tumor growth curves in the different groups after the indicated treatments. (n = 5). Scale bar: 1 cm. **P < 0.01, ***P < 0.001. F–I Representative plots of flow cytometric analysis and statistical analysis of the infiltration of CTLs (CD45⁺CD3⁺CD8⁺; F and G) and Treg cells (CD3⁺CD4⁺Foxp3⁺; H and I) in the distant tumors. (n = 5). Scale bar: 1 cm. *P < 0.05, **P < 0.01, ***P < 0.001