Skip to main content
Fig. 6 | Journal of Nanobiotechnology

Fig. 6

From: Self-amplification of oxidative stress with tumour microenvironment-activatable iron-doped nanoplatform for targeting hepatocellular carcinoma synergistic cascade therapy and diagnosis

Fig. 6

In vitro antitumour effect evaluation and mechanism exploration of the nanoplatform. a Flow cytometry of apoptosis levels in HepG2 cells incubated with PBS, DOX, HMON-Tf NPs, Fe-HMON-Tf NPs, DOX@HMON-Tf NPs, DOX@Fe-HMON-PEG NPs or DOX@Fe-HMON-Tf NPs for 24 h. b GSH levels in HepG2 cells incubated with PBS, DOX, HMON-Tf NPs, Fe-HMON-Tf NPs, DOX@HMON-Tf NPs, DOX@Fe-HMON-PEG NPs or DOX@Fe-HMON-Tf NPs for 24 h. **P < 0.01; ***P < 0.001, Kruskal–Wallis test; mean ± SD. c GPX4 activity in HepG2 cells incubated with PBS, DOX, HMON-Tf NPs, Fe-HMON-Tf NPs, DOX@HMON-Tf NPs, DOX@Fe-HMON-PEG NPs or DOX@Fe-HMON-Tf NPs for 24 h. ***P < 0.001; ****P < 0.0001, Kruskal–Wallis test; mean ± SD. d Western blot of key ferroptosis marker GPX4 expression and apoptosis markers NOX4 and Caspase-3 in HepG2 cells incubated with PBS, DOX, HMON-Tf NPs, Fe-HMON-Tf NPs, DOX@HMON-Tf NPs, DOX@Fe-HMON-PEG NPs or DOX@Fe-HMON-Tf NPs. e Proposed molecular mechanisms of nanoplatform-induced synergistic ferroptosis and apoptosis

Back to article page

Journal of Nanobiotechnology

ISSN: 1477-3155

Contact us