Fig. 3
A The cellular uptake of 4T1 cells after incubation with IR-780@MPDA for 8, 12 or 24 h. B The IR-780 median fluorescence intensity (MFI) at different incubation times. C Intracellular ROS generation in control, MPDA, IR-780, and IR-780@MPDA groups under laser irradiation (808 nm, 1 W/cm2, 300 s) as indicated. D The MFI of ROS in each treatment group. E Relative cell viability of 4T1 cells after incubation with different concentrations of MPDA or IR-780@MPDA in dark. F Relative cell viability of 4T1 cells under laser irradiation after incubation with different concentrations of MPDA, IR-780 or IR-780@MPDA as indicated. Asterisk * indicated p < 0.05. G Western blot analysis of CRT expression in vitro. H Detection of secreted extracellular ATP. I Detection of the released extracellular HMGB1