Fig. 4

Transcytosis efficiency in the BBB and BBTB models. Cell uptake of different preparations by C6 glioma cells in the bEnd.3/C6 in vitro BBB model (A) and in the HUVEC/C6 BBTB model (B). The fluorescence intensity of the lower chamber and cell viability in the BBB (C, D) and BBTB (E, F) models. (Hoechst 33,258 (blue), DiI (red); ×40 magnification; **p < 0.01, ***p < 0.001)