Fig. 1

Characterization of conformation-specific antibodies for Aβ₄₂Ms and Aβ₄₂Os. a Confocal fluorescence images of mouse 5xFAD brain sections using Cy3-labeled anti-Aβ₄₂ monoclonal antibody 1F12 or 2C6 and thioflavin S. (Scale bar: 500 μm). b Parallel analysis of the morphology of Aβ₄₂Ms and Aβ₄₂Os by cryo-transmission electron microscopy. (Scale bar: 500 nm). The SDS-PAGE (c) and Western blotting (d) analysis of the prepared Aβ₄₂Ms and Aβ₄₂Os using 1F12 or 2C6. The binding selectivity of 1F12 (e) and 2C6 (f) to Aβ₄₂ and Aβ₄₀ was determined by competitive ELISA. g The logIC50 value of 1F12 or 2C6 to Aβ₄₂ and Aβ₄₀. The data are presented as means ± SD, n = 3 in e and f. One-way analysis of variance (ANOVA) was used for multigroup comparisons. Statistical significance is indicated in the figures by ****p < 0.0001