Fig. 2

Characterization of AuNP–1F12 conjugates. a TEM images of synthetic AuNP. (Scale bar: 200 nm). b The size distributions of AuNP before and after modification. c A 12% reduced SDS-PAGE gel analysis of AuNP–1F12 and 1F12. d ELISA was used to determine the bioactivity of AuNP–1F12 conjugates. The optimal volume of K₂CO₃ for the conjugation of 1F12 with AuNP was evaluated by the color (e) and OD₅₂₅ value (g). The color (f) and OD₅₂₅ value (h) were used to evaluate the optimal concentration of 1F12 of synthetic AuNP-1F12. For Fig. 3b, d, g, and h, the data are presented as mean ± SD, n = 3. Statistical significance is indicated in the figures by n.s. (indicating no significance)