Fig. 4
Simultaneous detection of Aβ42Ms and Aβ42Os in the blood of 3- and 9-month-old 5xFAD and C57BL/6 J mice. The visual results (a) and grey value (b, c) on the T-line of the dLFI in blood samples from 5xFAD (n = 8) and C57BL/6 J (n = 6) mice at 3 and 9 months old. d, e Correlation between blood Aβ42Os level and Aβ plaque area (d, p < 0.0001) or soluble Aβ42 level (e, p < 0.0001) in the brain of 5xFAD mice, a total of n = 25, of which n = 5 in each age group, including 1, 3, 6, 9, and 12 months old. f Confocal fluorescence images of Aβ plaques and Iba 1-positive cell staining in 3 or 9-month-old 5xFAD mice. (Scale bar: 100 μm). Data are presented as means ± SD. Unpaired t-test was used for two-group comparisons. Statistical significance is indicated in the figures by **p < 0.01, ***p < 0.001 and n.s. (indicating no significance)