Fig. 3

In vitro cellular uptake of PCM/DiD and MM@PCM/DiD by RAW 264.7 macrophages and ECs. Nuclei were stained with DAPI (blue), whereas nanoparticles were stained with DiD (red). a CLSM images of PCM/DiD and MM@PCM/DiD phagocytosed by RAW 264.7 cells at 1 h or 3 h (scale bar = 40 µm). b FACS images and c quantification anilysis of PCM/DiD and MM@PCM/DiD phagocytosed by RAW 264.7 cells at 1 h or 3 h (n = 3, mean ± SD). d CLSM images of PCM/DiD and MM@PCM/DiD cellular uptake by ECs without or with TNF-α activated at 1 h or 3 h (scale bar = 40 µm). e, f FACS images of PCM/DiD and MM@PCM/DiD cellular uptake by ECs without or with TNF-α activated at 1 h or 3 h. g Quantification of cellular uptake of PCM/DiD and MM@PCM/DiD in ECs without or with TNF-α activated at 1 h or 3 h (n = 3, mean ± SD). Significances among the groups were determined by one-way ANOVA, followed by post hoc pairwise comparisons with the Tukey or Dunn honest significant difference. *p < 0.05, **p < 0.01, and ***p < 0.001. ns no significance