Fig. 3

ICG@PM@NP improved the efficacy of mild-PTT and decreased PD-L1 expression in vitro. A Representative fluorescence image of ICG@PM@NP accumulation in CT26 cells, scale bar = 50 μm. B, C Cell viability of CT26 cells detected by CCK-8 assay (n = 3). D Representative immunofluorescence images of ICG@PM@NP mediated CRT exposure after mild-PTT treatment, scale bar = 50 µm. E Quantification of ICG@PM@NP mediated CRT exposure after mild-PTT treatment (n = 3). F–G Effects of ICG@PM@NP on the basal PD-L1 protein expression in CT26 and 4T1 cells detected by western blot assay after treatments for 24 h (n = 3). H, I Quantification of PD-L1 protein expression levels in western blot assay by ImageJ in CT26 and 4T1 cells (n = 3). J, K Effects of ICG@PM@NP on the IFN-γ induced over-expression of PD-L1 protein in CT26 and 4T1 cells after treatments for 24 h (n = 3). L, M Quantification of the PD-L1 protein expression levels by ImageJ in CT26 and 4T1 cells (n = 3). Data were demonstrated as mean ± SD. Statistical analysis was performed via the two-tail Student’s t-test. ND, no significant difference; * p < 0.05; ** p < 0.01; *** p < 0.001