Fig. 4

ICG@PM@NP effectively inhibited tumor cell migration and metastasis. A, B Effects of ICG@PM@NP on the TGF-β, E-cadherin, and Vimentin protein expression in 4T1 and A549 cells detected by western blot assay after different treatments for 24 h (n = 3). C–H Quantification of TGF-β, E-cadherin, and Vimentin protein expression levels in western blot by ImageJ in 4T1 cells and A549 cells (n = 3). I–K Representative images and quantitative analysis of the migration of 4T1 cells and A549 cells after different treatments for 24 h (n = 3), scale bar = 200 µm. Data were demonstrated as mean ± SD. Statistical analysis was performed via the two-tail Student’s t-test. * p < 0.05; ** p < 0.01; *** p < 0.001