Fig. 6
Effects of ICG@PM@NP on PD-L1 protein expression, T cell infiltration, and cell apoptosis in vivo. A Representative in vivo infrared thermal images of CT26 tumors irradiated 60 s at 1 W/cm2 for twice at 24 h after different treatments. B The temperature curves of CT26 tumors after NIR laser irradiation for different time, the mice were pre-treated with different drugs for 24 h (n = 3). C, D PD-L1 expression detection and quantification in CT26 tumors by western blot (n = 3). E Representative fluorescence images of CD3+ T cells, CD8+ T cells, and CD4+ T cells in CT26 tumor slices to evaluate the effects of ICG@PM@NP mediated mild-PTT on T cell infiltration, scale bar = 50 µm. F Representative images of H&E staining, Ki67 staining, and Tunel staining, scale bar = 100 µm. G–H Quantification of Tunel+ and Ki67+ tumor cells (n = 3). Data were demonstrated as mean ± SD. Statistical analysis was performed via the two-tail Student’s t-test. * p < 0.05; ** p < 0.01; *** p < 0.001