Fig. 1

Identification of N-EVs and H-EVs. A Flow cytometric analysis showed the levels of apoptosis in N-OM-MSCs and H-OM-MSCs. Both apoptosis rates are less than 0.5% and there are no significant difference. (n = 5). (ns: no significance). B Western blot detected the expression of positive markers (TSG101, CD63, nad CD81) and negative markers (Calnexin) in H-EVs and N-EVs. C Morphological characteristic of the H-EVs and N-EVs were analyzed using TEM images, Bar = 100 nm. D Size distribution analysis of the H-EVs and N-EVs, analyzed by nanoparticle tracking assay (NTA). E The BCA assay quantified the protein concentration of H-EVs and N-EVs secreted by equal cells. (n = 3).*P < 0.05. F, G The schematic representation of EVs uptake experimental design and fluorescence image of PKH67-labeled H-EVs and N-EVs uptake by HBMECs, Bar: upper, 200 μm; lower, 50 μm.