Fig. 6
Staining of air pouch tissues on day 4. a H&E and b Masson trichrome staining of skin tissue from air pouches. Arrows point to the fibrous layer. c Thickness of the fibrous layer. d Numbers of infiltrating cells. Fibrous layer thickness and inflammatory cell infiltration were decreased in the LPS + M-Se@SiO2 group compared with the LPS and LPS + Se@SiO2 groups. e Cytokines in air pouch exudates evaluated by ELISA. f Immunofluorescence staining of skin tissue from air pouches. CCR7 (green), ARG1 (red), and DAPI (blue; nuclei). Arrows point to the fibrous layer (*, #, and & represent P < 0.05 compared with the control, LPS, and LPS + Se@SiO2 groups, respectively)