Fig. 3

gCpG promotes tumor specific Th1 cytokines production on CD4⁺ and CD8⁺ T cells. a Intracellular staining of IFNγ production on CD8⁺ and CD4⁺ T cells from splenocytes stimulated with OVA. b Intracellular staining of TNFα expression on CD8⁺ and CD4⁺ T cells from splenocytes stimulated with OVA. c Intracellular staining of IFNγ production on CD8⁺ and CD4⁺ T cells from TILs stimulated with OVA. d Intracellular staining of TNFα expression on CD8⁺ and CD4⁺ T cells from TILs stimulated with OVA. The assays were done in quadruplicates. The data shown are the representative of three experiments. *p < 0.05 and **p < 0.01, ***p < 0.001 and ****p < 0.0001